Characterization of stable hypoxia-preconditioned dental pulp stem cells compared with mobilized dental pulp stem cells for application for pulp regenerative therapy

Abstract Background Dental pulp stem cells (DPSCs) have been developed as a potential source of mesenchymal (MSCs) for regeneration dental and other tissues. However, further strategies to isolate highly functional DPSCs beyond the colony-forming methods are required. We demonstrated safety efficacy isolated by G-CSF-induced mobilization cultured under normoxia (mobilized DPSCs, MDPSCs) regeneration. The device isolation MDPSCs, however, is not cost-effective requires prolonged cell culture period. It well known that MSCs hypoxic-preconditions improved MSC proliferation activity stemness. Therefore, in this investigation, we attempted improve clinical utility hypoxia-preconditioned (hpDPSCs) compared with MDPSCs endodontic dentistry. Methods Colony-forming were preconditioned hypoxia stable closed system from individual dog teeth. examined rate, migration potential, anti-apoptotic activity, gene expression markers angiogenic/neurotrophic factors. Trophic effects conditioned medium (CM) also evaluated. In addition, immunomodulatory molecules upon stimulation IFN-γ was investigated. regenerative transplantation hpDPSCs assessed pulpectomized teeth dogs histological immunohistochemical analyses chemistry blood urine tests. Results higher rate major regulator oxygen homeostasis, HIF-1α , marker, CXCR-4 . direct migratory response G-CSF significantly than MDPSCs. CM stimulated neurite extension. there no changes angiogenic, migration, activities gene, PTGE upregulated IFN gamma difference nitric oxide observed. regenerated tissue quantitatively qualitatively similar hpDPSC transplants MDPSC There evidence toxicity or adverse events transplantation. Conclusions These results identical, although properties suggesting their